国家水生生物种质资源库国家斑马鱼资源中心 (China Zebrafish Resource Center)

ZKO number		ZKO358
Recent progress of this line		F1 individuals have been identified
Gene name		zgc:195282
Ensembl ID		ENSDARG00000079488
Zebrafish strain		AB
Cas9 target site 1 sequence		antisense strand GTGGCACTTCAAACACAGCggg
Cas9 target site 2 sequence
Position in the gene		Exon 2
Restriction enzyme (RE)		Msp A1l(CMG\|CKG)
PCR primer (forward)		CCGATGCCTTAGTGTTCTA
PCR primer (reverse)		CATTCATTCTCACCTCTTGG
Length of expected PCR product (bp)		418
Length of expected RE digestion product (bp)		200+417
Plasmids used for making Cas9 mRNA		pXT7-hCAS9
Plasmids?used?for?making?gRNA		pMD19T-gRNA
Primers?used?for?making?gRNA		Pr-ZKO 358
Dosage of injected Cas9 mRNA (ng/ul)		300
Dosage of injected gRNA (ng/ul)		30
Survival rate of F0 embryo @ 1dpf (%)		80
Method to estimate mutation efficiency		Restriction enzyme digestionRE digestion (Msp A1l)
Stage of the embryos collected for mutation detection		http://www.zfish.cn/upload/file/ZKOPDF/ZKO358.pdf
Mutation efficiency in F0 embryos (%)		25
Picture?from?F₀?embryos ? (gel?picture?or?sequencing?picture)		/upload/2014-09-18/635466696338360039.png 358
Mutation type		Indel
Germline transmission efficiency in adult F₀		28%(2/7)
Wild-type (WT) sequence		GTGGCACTTCAAACACAGC
Germline mutations in selected F0 (By screening F1 embryos)	F0-1
Germline mutations in selected F0 (By screening F1 embryos)	F0-2
Mosaicism in selected F0 (By screening F1 adults)	F0-1	GTGGCACTTCAAACACAAAGC(+2bp)
Mosaicism in selected F0 (By screening F1 adults)	F0-2
Allele name and predicted product	358a 358b	GTGGCACTTCAAACACAAAGC(30aa)
Allele name and predicted product	358a 358b
突变创建时间		2014-03-18
填表时间		2013/12/18
修改时间		2016/8/8
PI/负责人 (工作单位)

Genotype Information: